1QMJ
CG-16, a homodimeric agglutinin from chicken liver
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1999-02-15 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.380, 82.600, 112.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.150 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sla |
RMSD bond length | 0.007 |
RMSD bond angle | 1.477 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.250 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.056 * | 0.204 * |
Number of reflections | 15244 | |
<I/σ(I)> | 7.8 | 4 |
Completeness [%] | 87.8 | 75 |
Redundancy | 4.2 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.2 * | 22 * | drop consists of equal volume of protein and precipitant solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | sodium phosphate | 5 (mM) | |
3 | 1 | drop | 0.2 (M) | ||
4 | 1 | drop | lactose | 0.1 (M) | |
5 | 1 | drop | beta-mercaptoethanol | 4 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 2 (M) | precipitant |
7 | 1 | reservoir | isopropanol | 5 (%(v/v)) | precipitant |
8 | 1 | reservoir | beta-mercaptoethanol | 1 (%) | precipitant |