1QM7

X-ray structure of a three-fingered chimeric protein, stability of a structural scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	58.480, 58.480, 62.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.202
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FASCICULIN 2
RMSD bond length	0.023
RMSD bond angle	2.866
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.063 *	0.335 *
Number of reflections	7312
<I/σ(I)>	22.3	2.8
Completeness [%]	97.0	41 *
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.6		pH 4.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	sodium acetate	0.1 (M)