1QM5
Phosphorylase recognition and phosphorylysis of its oligosaccharide substrate: answers to a long outstanding question
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.410, 61.500, 132.380 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.102 | |
| Total number of observations | 152192 * | |
| Number of reflections | 72681 | |
| Completeness [%] | 80.0 | 64.5 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | (26-32)% PEG 4000, (0.1-0.6)M LICL, 0.1M TRIS/HCL PH8.5, 18 DEG C, HANGING DROP, 10MG/ML PROTEIN, 20MM GSG4, 50MM PHOSPHATE, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | GSG4 | ||
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | PEG4000 | 26-32 (%) | |
| 5 | 1 | reservoir | 0.1-0.6 (M) | ||
| 6 | 1 | reservoir | Tris-HCl | 1 (M) |
