1QM5

Phosphorylase recognition and phosphorylysis of its oligosaccharide substrate: answers to a long outstanding question

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	107.410, 61.500, 132.380
Unit cell angles	90.00, 105.03, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.228
Rwork	0.228
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	X-PLOR (3.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.102
Total number of observations	152192 *
Number of reflections	72681
Completeness [%]	80.0	64.5
Redundancy	2.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	(26-32)% PEG 4000, (0.1-0.6)M LICL, 0.1M TRIS/HCL PH8.5, 18 DEG C, HANGING DROP, 10MG/ML PROTEIN, 20MM GSG4, 50MM PHOSPHATE, pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	GSG4
3	1	drop		50 (mM)
4	1	reservoir	PEG4000	26-32 (%)
5	1	reservoir		0.1-0.6 (M)
6	1	reservoir	Tris-HCl	1 (M)