1QLM
The crystal structure of methenyltetrahydromethanopterin cyclohydrolase from the hyperthermophilic archaeon Methanopyrus kandleri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Detector | R-AXIS IIC |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 125.900, 125.900, 172.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.21900 |
Structure solution method | MIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 25.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (0.3) |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.067 | 0.280 |
Number of reflections | 54396 | |
<I/σ(I)> | 10.3 | 3.1 |
Completeness [%] | 98.8 | 98.7 |
Redundancy | 3.1 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4 | pH 4.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | reservoir | PEG1000 | 28 (%) | |
4 | 1 | reservoir | glycerol | 30 (%) | |
5 | 1 | reservoir | sodium acetate | 0.1 (M) |