1QLM
The crystal structure of methenyltetrahydromethanopterin cyclohydrolase from the hyperthermophilic archaeon Methanopyrus kandleri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Detector | R-AXIS IIC |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 125.900, 125.900, 172.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.21900 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 25.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.280 |
| Number of reflections | 54396 | |
| <I/σ(I)> | 10.3 | 3.1 |
| Completeness [%] | 98.8 | 98.7 |
| Redundancy | 3.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 | pH 4.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | reservoir | PEG1000 | 28 (%) | |
| 4 | 1 | reservoir | glycerol | 30 (%) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
