1QLG
Crystal structure of phytase with magnesium from Bacillus amyloliquefaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 296 |
Detector technology | IMAGE PLATE |
Detector | MACSCIENCE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.060, 64.040, 105.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.200 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.26500 |
Structure solution method | DIRECT METHODS |
Starting model (for MR) | 1poo |
RMSD bond length | 0.008 |
RMSD bond angle | 1.513 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.084 | 0.317 |
Number of reflections | 17305 | |
<I/σ(I)> | 13.5 | 2.62 |
Completeness [%] | 93.0 | 87.2 |
Redundancy | 1.07 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 277 * | drop consists of equal volume of protein and precipitant solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | reservoir | MPD | 20 (%) | precipitant |
3 | 1 | reservoir | MES | 0.1 (M) | precipitant |