1QLB
respiratory complex II-like fumarate reductase from Wolinella succinogenes
Experimental procedure
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 275 |
Detector technology | CCD |
Collection date | 1999-03-26 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 118.400, 85.050, 188.850 |
Unit cell angles | 90.00, 96.46, 90.00 |
Refinement procedure
Resolution | 38.870 - 2.330 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1QLA |
RMSD bond length | 0.007 |
RMSD bond angle | 21.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.870 | 2.390 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.075 * | 0.388 * |
Number of reflections | 152939 | |
<I/σ(I)> | 9.5 | 2.8 |
Completeness [%] | 95.8 | 99.7 |
Redundancy | 2.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.4 * | pH 6.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9.5 (mg/ml) | |
10 | 1 | drop | malonate | 1 (mM) | |
11 | 1 | drop | HEPES | 10 (mM) | |
12 | 1 | drop | citrate | 10 (mM) | |
13 | 1 | reservoir | 150 (mM) | ||
14 | 1 | reservoir | PEG3350 | 10 (%) | |
15 | 1 | reservoir | citrate | 20 (mM) | |
2 | 1 | drop | dodecyl-beta-maltoside | 0.05 (%) | |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | drop | decylmaltoside | 0.20 (%) | |
5 | 1 | drop | benzamidine | 2.4 (%) | |
6 | 1 | drop | PEG3350 | 10 (%) | |
7 | 1 | drop | 75 (mM) | ||
8 | 1 | drop | DMF | 5 (%) | |
9 | 1 | drop | DMN | 1 (mM) |