1QL8
FACTOR XA SPECIFIC INHIBITOR IN COMPLEX WITH BOVINE TRYPSIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-15 |
| Detector | R-AXIS IV |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 125.810, 125.810, 125.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| Structure solution method | OTHER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.147 | 0.543 |
| Number of reflections | 7494 | |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 0.1M IMIDAZOLE PH7.0, 0.3M AMMONIUM SULPHATE, 30% PEG8K, pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | inhibitor | 2 (mg/ml) | |
| 3 | 1 | reservoir | imidazole | 0.1 (M) | pH7 |
| 4 | 1 | reservoir | ammonium sulfate | 0.3 (M) | |
| 5 | 1 | reservoir | PEG8000 | 30 (%) |
