1QL7

FACTOR XA SPECIFIC INHIBITOR IN COMPLEX WITH BOVINE TRYPSIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1998-07-15
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	54.060, 58.580, 63.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.203 *
Rwork	0.200
Structure solution method	OTHER
RMSD bond length	0.008
RMSD bond angle	24.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.111	0.425
Number of reflections	13570
<I/σ(I)>	8.3
Completeness [%]	96.1	93.3
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8		0.1M IMIDAZOLE PH8.0, 0.1M AMMONIUM SULPHATE, 20% PEG8K, pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	inhibitor	2 (mg/ml)
3	1	reservoir	imidazole	0.1 (M)	pH8.
4	1	reservoir	ammonium sulfate	0.1 (M)
5	1	reservoir	PEG8000	20 (%)