1QL6
THE CATALYTIC MECHANISM OF PHOSPHORYLASE KINASE PROBED BY MUTATIONAL STUDIES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.560, 68.171, 112.475 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| Rwork | 0.240 |
| R-free | 0.33000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2phk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.042 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.400 | |
| Rmerge | 0.048 * | 0.221 * |
| Total number of observations | 40795 * | |
| Number of reflections | 12745 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 86.6 | 78.5 * |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.2 * | 14 * | pH 6.90 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Phk gamma trnc | 10-12 (mg/ml) | |
| 10 | 1 | reservoir | HEPES/NaOH | 50 (mM) | |
| 11 | 1 | reservoir | 10 (mM) | ||
| 12 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 13 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 14 | 1 | reservoir | 0.02 (%(w/v)) | ||
| 2 | 1 | drop | AMPPNP | 3 (mM) | |
| 3 | 1 | drop | HEPES/NaOH | 10 (mM) | |
| 4 | 1 | drop | glycerol | 2 (%(v/v)) | |
| 5 | 1 | drop | dithiothreitol | 10 (mM) | |
| 6 | 1 | drop | 0.02 (%(w/v)) | ||
| 7 | 1 | drop | EDTA | 0.1 (mM) | |
| 8 | 1 | drop | 10 (mM) | ||
| 9 | 1 | reservoir | PEG8000 | 5 (%(w/v)) |
