1QL3
Structure of the soluble domain of cytochrome c552 from Paracoccus denitrificans in the reduced state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.690, 54.320, 140.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24200 |
| Structure solution method | OTHER |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.036 | 0.143 |
| Total number of observations | 277074 * | |
| Number of reflections | 80254 | |
| <I/σ(I)> | 30.9 | 8.4 |
| Completeness [%] | 98.5 | 98 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 18 * | pH 4.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | cytochrome | 1 (mM) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 5 | 1 | reservoir | PEG1500 | 20.0-22.5 (%) | |
| 6 | 1 | reservoir | glycerol | 10 (%(v/v)) |
