1QL1
INOVIRUS (FILAMENTOUS BACTERIOPHAGE) STRAIN PF1 MAJOR COAT PROTEIN ASSEMBLY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 1.000, 1.000, 1.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| Rwork | 0.300 |
| R-free | 0.38000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ifn |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.800 |
| Data reduction software | CCP13 ((LSQINT)) |
| Data scaling software | CCP13-FDSCALE |
| Refinement software | FXPLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 816 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 8.1 * | 4 * | Nave, C., (1981) J.Mol.Biol., 149, 675. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | virus | 0.5 (mg/ml) | |
| 2 | 1 | 2 | Tris-HCl | 10 (mM) |
