1QKX

Alpha-spectrin Src Homology 3 domain, N47A mutant in the distal loop.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Collection date	1999-03-15
Spacegroup name	P 21 21 21
Unit cell lengths	33.600, 42.240, 49.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000 *
R-factor	0.18 *
Rwork	0.216
R-free	0.22500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1shg
RMSD bond length	0.008
RMSD bond angle	1.356 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.090
Number of reflections	6033
Completeness [%]	87.1	75 *
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6		DROP CONTAINS 1.1 M AMM.SULPH., 90MM CITRIC BUFFERPH=6.0, 90MM BIS-TRIS PROPANE, 0.9MM EDTA AND 0.9MM DTT., pH 6.00