1QKX
Alpha-spectrin Src Homology 3 domain, N47A mutant in the distal loop.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Collection date | 1999-03-15 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.600, 42.240, 49.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 * |
R-factor | 0.18 * |
Rwork | 0.216 |
R-free | 0.22500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1shg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.356 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.880 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.090 | |
Number of reflections | 6033 | |
Completeness [%] | 87.1 | 75 * |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6 | DROP CONTAINS 1.1 M AMM.SULPH., 90MM CITRIC BUFFERPH=6.0, 90MM BIS-TRIS PROPANE, 0.9MM EDTA AND 0.9MM DTT., pH 6.00 |