1QKS
CYTOCHROME CD1 NITRITE REDUCTASE, OXIDISED FORM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID09 |
Synchrotron site | ESRF |
Beamline | ID09 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-03-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.400, 60.600, 100.200 |
Unit cell angles | 90.00, 112.30, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.280 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.20000 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 25.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.330 |
High resolution limit [Å] | 1.280 | 1.280 |
Number of reflections | 278611 | |
<I/σ(I)> | 17 | 8 |
Completeness [%] | 91.8 | 80.9 |
Redundancy | 2.2 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 15 * | 2.3 M AMMONIUM SULFATE, 50MM POTASSIUM PHOSPHATE, PH 7.0, AND CRYOPROTECTANT 15% GLYCEROL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2.2-2.3 (M) | |
3 | 1 | reservoir | potassium phosphate | 50 (mM) |