1QKK
Crystal structure of the receiver domain and linker region of DctD from Sinorhizobium meliloti
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-11-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98333, 0.98100, 0.98076 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 58.651, 58.769, 167.888 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.420 - 1.700 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.23100 * |
Structure solution method | MAD |
RMSD bond length | 0.008 * |
RMSD bond angle | 21.100 * |
Data reduction software | HKL-2000 ((DENZO)) |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.059 * | |
Number of reflections | 32439 * | |
<I/σ(I)> | 34 | 2.2 |
Completeness [%] | 86.1 * | 100 |
Redundancy | 8 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 | 294 * | 50 MM NA SUCCINATE PH 5.6, 70 MM AMMONIUM PHOSPHATE MONOBASIC, 1 MM DTT |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-7 (mg/ml) | |
2 | 1 | reservoir | succinate | 50 (mM) | |
3 | 1 | reservoir | 75 (mM) |