1QKE
ERABUTOXIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.840, 46.620, 21.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.000 - 1.500 |
| Structure solution method | RIGID BODY REFINEMENT |
| Starting model (for MR) | 5ebx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 17.670 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 1.620 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.042 * | |
| Total number of observations | 40456 * | |
| Number of reflections | 7978 | |
| <I/σ(I)> | 30.9 | 10.16 |
| Completeness [%] | 95.1 | 92.2 |
| Redundancy | 4.9 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 40 (%sat) | |
| 3 | 1 | reservoir | potassium phosphate | 0.05 (M) |
