1QKD

ERABUTOXIN

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1992-10
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	55.320, 53.540, 40.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.400 - 1.490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ebx
RMSD bond length	0.010
RMSD bond angle	18.130 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.400	1.600
High resolution limit [Å]	1.490	1.490
Rmerge	0.065 *	0.204 *
Number of reflections	19420
<I/σ(I)>	29.7	5.25
Completeness [%]	94.8	86
Redundancy	5.2	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2		pH 7.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	ammonium sulfate	40 (%sat)