1QKB

OLIGO-PEPTIDE BINDING PROTEIN (OPPA) COMPLEXED WITH KVK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	120
Detector technology	IMAGE PLATE
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	109.730, 75.780, 70.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.800
R-factor	0.181 *
Rwork	0.181
R-free	0.21528
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1OLB
RMSD bond length	0.010
RMSD bond angle	0.026
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.800
High resolution limit [Å]	1.750	1.750
Rmerge	0.063 *
Number of reflections	59716
<I/σ(I)>	6.5
Completeness [%]	92.2	59.6
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		CO-CRYSTALLIZED WITH URANIUM ACETATE, PH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000	5-10 (%(w/v))
2	1	drop	sodium acetate	50 (mM)
3	1	drop	uranyl acetate	1 (mM)
4	1	drop	peptide	30 (mg/ml)