1QJV
Pectin methylesterase PemA from Erwinia chrysanthemi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.694, 85.507, 96.690 |
| Unit cell angles | 90.00, 93.69, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.370 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.21100 * |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.230 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.5) |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.064 | 0.224 * |
| Number of reflections | 32934 | |
| <I/σ(I)> | 13.43 | 3 |
| Completeness [%] | 99.1 * | 82.6 * |
| Redundancy | 4.04 | 3.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | HANGING DROP AGAINST 2.0 M AMMONIUM SULFATE AND 0.1M MES BUFFER AT PH 6.8 THE PROTEIN CONCENTATION WAS ABOUT 3 MG./ML. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium sulfate | 1.6 (M) | |
| 2 | 1 | drop | MES | 0.1 (M) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | reservoir | MES | 0.1 (M) |
