1QJV
Pectin methylesterase PemA from Erwinia chrysanthemi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-27 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.694, 85.507, 96.690 |
Unit cell angles | 90.00, 93.69, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.370 |
R-factor | 0.17 |
Rwork | 0.170 |
R-free | 0.21100 * |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 1.230 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (0.5) |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.064 | 0.224 * |
Number of reflections | 32934 | |
<I/σ(I)> | 13.43 | 3 |
Completeness [%] | 99.1 * | 82.6 * |
Redundancy | 4.04 | 3.04 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | HANGING DROP AGAINST 2.0 M AMMONIUM SULFATE AND 0.1M MES BUFFER AT PH 6.8 THE PROTEIN CONCENTATION WAS ABOUT 3 MG./ML. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | ammonium sulfate | 1.6 (M) | |
2 | 1 | drop | MES | 0.1 (M) | |
3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
4 | 1 | reservoir | MES | 0.1 (M) |