1QJP
HIGH RESOLUTION STRUCTURE OF THE OUTER MEMBRANE PROTEIN A (OMPA) TRANSMEMBRANE DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.100, 79.700, 50.200 |
| Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.650 |
| Rwork | 0.155 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bxw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.061 * | 0.310 * |
| Number of reflections | 29702 | |
| <I/σ(I)> | 13.7 | 3.2 |
| Completeness [%] | 95.4 | 94 * |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 * | 10% PEG 8000, 10 % MPD, 25 MM KH2PO4 PH 5.1 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | C8E4 | 0.6 (%(w/v)) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
| 3 | 1 | reservoir | 2-propanol | 3.8 (%(v/v)) | |
| 4 | 1 | reservoir | C8E4 | 0.5 (%(w/v)) | |
| 5 | 1 | reservoir | hexyldimethylaminoxide | 1.8 (%(w/v)) | |
| 6 | 1 | reservoir | ammonium acetate | 0.1 (M) |
