1QJP
HIGH RESOLUTION STRUCTURE OF THE OUTER MEMBRANE PROTEIN A (OMPA) TRANSMEMBRANE DOMAIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-15 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 65.100, 79.700, 50.200 |
Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
Resolution | 12.000 - 1.650 |
Rwork | 0.155 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bxw |
RMSD bond length | 0.008 |
RMSD bond angle | 2.500 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.750 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.061 * | 0.310 * |
Number of reflections | 29702 | |
<I/σ(I)> | 13.7 | 3.2 |
Completeness [%] | 95.4 | 94 * |
Redundancy | 2.5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 * | 10% PEG 8000, 10 % MPD, 25 MM KH2PO4 PH 5.1 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | C8E4 | 0.6 (%(w/v)) | |
2 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
3 | 1 | reservoir | 2-propanol | 3.8 (%(v/v)) | |
4 | 1 | reservoir | C8E4 | 0.5 (%(w/v)) | |
5 | 1 | reservoir | hexyldimethylaminoxide | 1.8 (%(w/v)) | |
6 | 1 | reservoir | ammonium acetate | 0.1 (M) |