Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.350, 71.980, 131.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.000 |
R-factor | 0.21 * |
Rwork | 0.210 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 14-3-3 ZETA |
RMSD bond length | 0.012 |
RMSD bond angle | 0.033 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.059 | 0.320 |
Number of reflections | 37059 | |
<I/σ(I)> | 13.3 | 2.1 |
Completeness [%] | 83.5 | 41.6 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.2 | 4 * | used to seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MES | 100 (mM) | |
2 | 1 | reservoir | 20 (mM) | ||
3 | 1 | reservoir | isopropanol | 15-17 (%) | |
4 | 1 | reservoir | PEG4000 | 12 (%) |