1QJ8
CRYSTAL STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 82.400, 82.400, 206.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.000 - 1.900 |
| R-factor | 0.204 * |
| Rwork | 0.204 |
| R-free | 0.24600 |
| Structure solution method | MIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.034 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 * | 0.270 * |
| Number of reflections | 20466 | |
| <I/σ(I)> | 14.6 | 2.9 |
| Completeness [%] | 95.0 | 92 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | 30 % (V/V) 2-PROPANOL, 20 % (V/V) GLYCEROL, 0.2 M CACL2, 0.1 M NA-ACETATE PH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 2-propanol | 30 (%(v/v)) | |
| 2 | 1 | reservoir | glycerol | 20 (%(v/v)) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 5 | 1 | drop | protain | 20 (mg/ml) | |
| 6 | 1 | drop | 0.6 (%(v/v)) | ||
| 7 | 1 | drop | Tris-HCl | 20 (mM) |
