1QIZ

HUMAN INSULIN HEXAMERS WITH CHAIN B HIS MUTATED TO TYR COMPLEXED WITH RESORCINOL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	289
Detector technology	IMAGE PLATE
Collection date	1993-08-15
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 1 21 1
Unit cell lengths	60.810, 62.050, 47.650
Unit cell angles	90.00, 110.40, 90.00

Refinement procedure

Resolution	19.200 - 2.000
Rwork	0.191
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	R6 (NATIVE) INSULIN
RMSD bond length	0.011
RMSD bond angle	0.036
Data reduction software	DENZO
Data scaling software	SCALA
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.200	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.058	0.224
Number of reflections	21525
<I/σ(I)>	7	3
Completeness [%]	96.0	87.6
Redundancy	2.5	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	6.8		CRYSTALLISATION IN BATCH, 10 MG B5 TYR INSULIN DISSOLVED IN 2 ML 0.02M HCL. TO THIS ADDED 0.1 ML 0.12M ZINC ACETATE, 1.04 ML 0.2 M TRI-SODIUM CITRATE, 0.4 ML 5.0% (AQ.) PHENOL AND 120 MG NACL. PH ADJUSTED TO 6.5-7.8 .

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	insulin
2	1	1		0.02 (M)	2 ml
3	1	1	zinc acetate	0.12 (M)	2 ml
4	1	1	trisodium citrate	0.2 (M)	1.04 ml
5	1	1	resorcinol	5.0 (%)	0.4 ml