1QIU
A triple beta-spiral in the adenovirus fibre shaft reveals a new structural motif for biological fibres
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-09-05 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.510, 95.870, 211.770 |
| Unit cell angles | 90.00, 106.83, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qhv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 26.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS (0.4) |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.132 * | 0.287 * |
| Number of reflections | 103327 | 10696 * |
| <I/σ(I)> | 4.1 | 2.4 |
| Completeness [%] | 84.0 | 59.9 * |
| Redundancy | 2.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4 | 20 * | 0.8 M AMMONIUM SULPHATE, 100 MM SODIUM CITRATE, PH 4.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.8 (M) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) |
