1QIS

ASPARTATE AMINOTRANSFERASE FROM ESCHERICHIA COLI, C191F MUTATION, WITH BOUND MALEATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	297
Detector technology	IMAGE PLATE
Detector	RIGAKU IMAGE PLATE
Spacegroup name	C 2 2 21
Unit cell lengths	157.300, 85.100, 78.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.188
Rwork	0.188
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1asa
RMSD bond length	0.015
RMSD bond angle	24.400 *
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.038
Number of reflections	40823
Completeness [%]	90.0
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		PROTEIN SOLUTION: 13MG/ML PROTEIN, 20 MM POTASSIUM PHOSPHATE BUFFER, PH 7.5, 10 UM PLP, 5 MM EDTA, RESERVOIR SOLUTION: 20MM POTASSIUM PHOSPHATE BUFFER, PH 7.5, AND 45-50% AMMONIUM SULFATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13 (mg/ml)
2	1	drop	potassium phosphate	20 (mM)
3	1	drop	PLP	0.01 (mM)
4	1	drop	EDTA	5 (mM)
5	1	reservoir	ammonium sulfate	40-54 (%)
6	1	reservoir	potassium phosphate	20 (mM)
7	1	drop	inhibitor maleate	5 (mM)