1QI7
THE CRYSTAL STRUCTURE AT 2.0 A OF SAPORIN SO6, A RIBOSOME INACTIVATING PROTEIN FROM SAPONARIA OFFICINALIS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 309 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 67.530, 67.530, 119.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.000 |
Rwork | 0.182 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1paf |
RMSD bond length | 0.012 |
RMSD bond angle | 2.240 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.230 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.109 * | 0.294 |
Total number of observations | 210203 * | |
Number of reflections | 19206 | |
Completeness [%] | 98.5 | 96.7 |
Redundancy | 8.7 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.7 | 309 * | pH 4.7 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | reservoir solution | ||
3 | 1 | drop | PEG400 | 10 (%(w/v)) | |
4 | 1 | reservoir | ammonium sulfate | 2.8 (M) | |
5 | 1 | reservoir | sodium citrate | 0.1 (M) |