1QHY
CHLORAMPHENICOL PHOSPHOTRANSFERASE FROM STREPTOMYCES VENEZUELAE IN COMPLEX WITH ATPGAMMAS AND CHLORAMPHENICOL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 200.000, 200.000, 200.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qhn |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.600 | |
Rmerge | 0.061 | 0.367 * |
Total number of observations | 304201 * | |
Number of reflections | 21331 | |
<I/σ(I)> | 16.2 | |
Completeness [%] | 99.7 * | 99.8 * |
Redundancy | 14.26 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mM) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | drop | ammonium sulfate | 0.4 (M) | |
4 | 1 | drop | magnesium chloride | 10 (mM) | |
5 | 1 | drop | ATPgammaS | 10 (mM) | |
6 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
7 | 1 | reservoir | ammonium sulfate | 1.14 (M) |