1QHX
CHLORAMPHENICOL PHOSPHOTRANSFERASE IN COMPLEX WITH ATP FROM STREPTOMYCES VENEZUELAE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 200.000, 200.000, 200.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 - 2.500 |
| R-factor | 0.213 |
| Rwork | 0.228 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qhn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.200 | |
| Rmerge | 0.058 * | 0.177 * |
| Total number of observations | 327116 * | |
| Number of reflections | 23682 * | |
| <I/σ(I)> | 22.4 | |
| Completeness [%] | 99.1 * | 99.5 * |
| Redundancy | 13.81 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 0.4 (M) | |
| 4 | 1 | drop | acetonitrile | 4 (%) | |
| 5 | 1 | drop | ATP | 10 (mM) | |
| 6 | 1 | drop | magnesium chloride | 10 (mM) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 8 | 1 | reservoir | ammonium sulfate | 1.14 (M) |
