1QHT
DNA POLYMERASE FROM THERMOCOCCUS SP. 9ON-7 ARCHAEON
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 98 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10-15 |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.300, 98.700, 112.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.250 * |
R-factor | 0.245 |
Rwork | 0.245 |
R-free | 0.30800 * |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.260 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.055 * |
Total number of observations | 652420 * |
Number of reflections | 58956 |
Completeness [%] | 94.2 |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 9 | 291 | MICRODIALYSIS AT 291 K: 4-7% PEG 1500, 10 MM NICL2, 100 MM GLYCINE-NAOH, PH 9.0 OR 9.1; 25 MICROLITERS OF 3.9 MG/ML PROTEIN |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 3.9 (mg/ml) | |
2 | 1 | 2 | glycine-NaOH | 100 (mM) | |
3 | 1 | 2 | 10 (mM) | ||
4 | 1 | 2 | PEG1500 | 4-7 (%) |