1QHS
CHLORAMPHENICOL PHOSPHOTRANSFERASE IN COMPLEX WITH CHLORAMPHENICOL FROM STREPTOMYCES VENEZUELAE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 200.000, 200.000, 200.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 - 2.800 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qhn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.098 |
| Number of reflections | 20175 |
| <I/σ(I)> | 12.1 |
| Completeness [%] | 93.0 |
| Redundancy | 27.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 0.4 (M) | |
| 4 | 1 | drop | acetonitrile | 4 (%) | |
| 5 | 1 | drop | Cm | 2 (mM) | |
| 6 | 1 | drop | magnesium chloride | 10 (mM) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 8 | 1 | reservoir | ammonium sulfate | 1.14 (M) |
