1QHL

CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF MUKB AT 2.2A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-04-01
Detector	MARRESEARCH
Wavelength(s)	0.91, 0.9790, 0.9797, 1.2
Spacegroup name	P 6 2 2
Unit cell lengths	111.400, 111.400, 65.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.217 *
Rwork	0.217
R-free	0.29200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	28.600 *
Data reduction software	MOSFLM (V. 6.0)
Data scaling software	CCP4 ((SCALA))
Phasing software	CCP4
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.091	0.263
Number of reflections	382097
<I/σ(I)>	5	2.9
Completeness [%]	99.6	97.3
Redundancy	13.1	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.9	4 *	0.1M NA-CITRATE PH 4.9, 18-22% PEG 600, 50MG/ML, DROPS 1+1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	0.1 (M)
2	1	reservoir	PEG600	18-22 (%)
3	1	drop	protein	50 (mg/ml)