1QHH
STRUCTURE OF DNA HELICASE WITH ADPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS |
Synchrotron site | SRS |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 65 |
Unit cell lengths | 138.936, 138.936, 111.642 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.228 * |
Rwork | 0.228 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 3.047 |
Data reduction software | DENZO |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.103 |
Number of reflections | 42105 |
Completeness [%] | 92.5 |
Redundancy | 2.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * | Bird, L.E., (1998) Nucl. Acids Res., 26, 2686. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris | 50 (mM) | |
3 | 1 | drop | 300 (mM) | ||
4 | 1 | reservoir | MES | 100 (mM) | |
5 | 1 | reservoir | sodium acetate | 1.0 (M) |