1QHB
VANADIUM BROMOPEROXIDASE FROM RED ALGA CORALLINA OFFICINALIS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1997-06 |
Detector | MARRESEARCH |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 201.915, 201.915, 178.109 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
Rwork | 0.172 |
R-free | 0.22700 |
Structure solution method | MIRAS |
RMSD bond length | 0.012 |
RMSD bond angle | 0.031 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (WITH INPUT DM PHASES) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.157 | 0.513 |
Total number of observations | 491505 * | |
Number of reflections | 156862 * | |
<I/σ(I)> | 6.6 | 2.3 |
Completeness [%] | 96.8 | 95.2 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 | 291 * | 2M DIHYDROGEN PHOSPHATE, 0.1 M TRIS-HCL BUFFER, FINAL PH 5. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | reservoir | ammonium dihydrogen phosphate | 2 (M) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) |