1QH0
FERREDOXIN:NADP+ REDUCTASE MUTANT WITH LEU 76 MUTATED BY ASP AND LEU 78 MUTATED BY ASP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM02 |
Synchrotron site | ESRF |
Beamline | BM02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-12 |
Spacegroup name | P 65 |
Unit cell lengths | 86.940, 86.940, 96.590 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 1.900 * |
R-factor | 0.21 * |
Rwork | 0.210 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1que |
RMSD bond length | 0.007 |
RMSD bond angle | 26.730 * |
Data reduction software | XDS |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.100 * | 2.040 |
High resolution limit [Å] | 1.900 * | 1.930 |
Rmerge | 0.089 | 0.181 |
Number of reflections | 28625 | |
<I/σ(I)> | 5.3 | 3.9 |
Completeness [%] | 92.0 | 58 |
Redundancy | 4.2 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 20 * | pH 5.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.75 (mM) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
3 | 1 | drop | beta-octylglucoside | 5 (%(w/v)) | unbuffered |
4 | 1 | reservoir | PEG6000 | 18-20 (%(w/v)) | |
5 | 1 | reservoir | ammonium sulfate | 20 (mM) | |
6 | 1 | reservoir | MES- NaOH | 0.1 (M) | pH5.0 |