1QGX

X-RAY STRUCTURE OF YEAST HAL2P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	54.680, 44.910, 71.600
Unit cell angles	90.00, 111.08, 90.00

Refinement procedure

Resolution	67.000 - 1.600
Rwork	0.197
R-free	0.23500
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	0.025
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.000
High resolution limit [Å]	1.600	1.600 *
Rmerge	0.073 *	0.391 *
Number of reflections	42901
<I/σ(I)>	8.2
Completeness [%]	99.7 *	100 *
Redundancy	5.1	3.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop		5 (mM)
3	1	drop	PAP	0.6 (mM)
4	1	reservoir	mPEG5000	30 (%(w/v))
5	1	reservoir	sodium acetate	0.1 (M)
6	1	reservoir	beta-mercaptoethanol	5 (mM)
7	1	reservoir	MES	0.1 (M)