1QGN
CYSTATHIONINE GAMMA-SYNTHASE FROM NICOTIANA TABACUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 120.000, 129.500, 309.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.900 |
| R-factor | 0.201 * |
| Rwork | 0.201 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cs1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.148 | 0.554 * |
| Total number of observations | 473805 * | |
| Number of reflections | 95418 | |
| <I/σ(I)> | 6.9 | 1.8 |
| Completeness [%] | 88.2 * | 86.4 * |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.8 * | 100 MM MES/NAOH, PH6.5, 200 MM MGAC2, 12 % (W/V) PEG8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES-NaOH | 100 (mM) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | PEG8000 | 12 (%(w/v)) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | |
| 5 | 1 | drop | PLP | 0.010 (mM) |
