1QGI
CHITOSANASE FROM BACILLUS CIRCULANS
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 293 |
Wavelength(s) | 1.0000,1.0721,1.0722,1.0728 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.300, 128.000, 57.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 1.600 |
Rwork | 0.192 |
R-free | 0.23500 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 22.000 * |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.600 | |
Rmerge | 0.085 | 0.656 * |
Total number of observations | 462029 * | |
Number of reflections | 40769 | |
Completeness [%] | 95.1 | 83.7 * |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.2 | 293 * | pH 7.2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | ammonium sulfate | 65 (%(w/v)) | |
2 | 1 | reservoir | ammonium sulfate | 75 (%(w/v)) |