1QG2
CANINE GDP-RAN R76E MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-21 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 102.320, 102.320, 42.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.198 * |
| Rwork | 0.174 |
| R-free | 0.24600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EBI-1279 |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 2.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.112 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.066 | 0.343 |
| Number of reflections | 9871 | |
| <I/σ(I)> | 9.4 | 2.1 |
| Completeness [%] | 95.5 | 91.9 |
| Redundancy | 2.5 * | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 18 * | SEE PUBLICATION, pH 7.20 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG1000 | 20-36 (%(v/v)) | |
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 50 (mM) |
