1QG0

WILD-TYPE PEA FNR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Spacegroup name	P 21 21 21
Unit cell lengths	73.900, 94.100, 140.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
Rwork	0.218
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1FNB
RMSD bond length	0.015
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	33119
<I/σ(I)>	16.7	2.9
Completeness [%]	93.5	76.1
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.2		pH 7.2