1QFT

HISTAMINE BINDING PROTEIN FROM FEMALE BROWN EAR RHIPICEPHALUS APPENDICULATUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Spacegroup name	P 21 21 21
Unit cell lengths	77.420, 74.790, 78.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.250
R-factor	0.187 *
Rwork	0.184
R-free	0.20100 *
Structure solution method	MIR
RMSD bond length	0.011 *
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.250
Rmerge	0.184 *
Number of reflections	108735
Completeness [%]	86.3
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	MES	0.1 (M)
3	1	reservoir	cobalt chloride hexahydrate	0.01 (M)
4	1	reservoir	ammonium sulfate	1.8 (M)