1QFP

N-TERMINAL DOMAIN OF SIALOADHESIN (MOUSE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	38.900, 38.900, 152.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.198 *
Rwork	0.198
R-free	0.27600
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	25.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.970
High resolution limit [Å]	2.800	2.800
Rmerge	0.067
Number of reflections	3275
Completeness [%]	89.7	89.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.6 *		16 % (W/V) PEG 4000, 8% (V/V) PROPAN-2-OL, 10 MM DTT, 80MM SODIUM HEPES PH 7.5, 5MG/ML PROTEIN

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	3' sialyllactose	25 (mM)
3	1	reservoir	PEG4000	30 (%(w/v))
4	1	reservoir	sodium citrate	0.1 (M)
5	1	reservoir	ammonium acetate	0.2 (M)