1QFH

DIMERIZATION OF GELATION FACTOR FROM DICTYOSTELIUM DISCOIDEUM: CRYSTAL STRUCTURE OF ROD DOMAINS 5 AND 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	43.448, 103.150, 124.361
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.630 - 2.200
R-factor	0.2192
Rwork	0.222
R-free	0.26700
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	0.019
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.230	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.048	0.091
Total number of observations	82513 *
Number of reflections	28551 *	4076 *
<I/σ(I)>	8.8	6.9
Completeness [%]	98.0	97.6
Redundancy	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.4 *	20 *	30% PEG 1000, 0.1M TRIS_HCL PH 7.2, 25% GLYCEROL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	2.6 (mg/ml)
2	1	2	potassium phosphate	20 (mM)
3	1	2		100 (mM)