1QFG
E. COLI FERRIC HYDROXAMATE RECEPTOR (FHUA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-08-01 |
Detector | MARRESEARCH |
Spacegroup name | P 61 |
Unit cell lengths | 171.550, 171.550, 87.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.300 - 2.500 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.27100 |
RMSD bond length | 0.026 |
RMSD bond angle | 28.300 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.300 | 2.500 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.078 | 0.334 |
Number of reflections | 53749 | |
<I/σ(I)> | 17 | |
Completeness [%] | 99.3 | 92.2 |
Redundancy | 6.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 290 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6.5 (mg/ml) | |
2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
3 | 1 | reservoir | mPEG2000 | 11 (%) | |
4 | 1 | reservoir | glycerol | 20 (%) | |
5 | 1 | reservoir | PEG200 | 3 (%) | |
6 | 1 | reservoir | dimethyldecylamine-N-oxide | 0.8 (%) | |
7 | 1 | reservoir | cis-inositol | 1 (%) |