1QEZ
SULFOLOBUS ACIDOCALDARIUS INORGANIC PYROPHOSPHATASE: AN ARCHAEL PYROPHOSPHATASE.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 294 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.120, 98.730, 107.250 |
| Unit cell angles | 90.00, 78.01, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| Rwork | 0.197 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2prd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.091 * | 0.280 * |
| Total number of observations | 256303 * | |
| Number of reflections | 38004 | |
| <I/σ(I)> | 10.1 | 1.8 |
| Completeness [%] | 93.8 | 62.2 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | 26 * | protein solution is mixed 2:1 with well solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 21-26 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 3.5-4.5 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) |
