1QEX

BACTERIOPHAGE T4 GENE PRODUCT 9 (GP9), THE TRIGGER OF TAIL CONTRACTION AND THE LONG TAIL FIBERS CONNECTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1997-06
Detector	RIGAKU
Spacegroup name	H 3 2
Unit cell lengths	94.330, 94.330, 440.940
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.23 *
Rwork	0.239
R-free	0.27700
Structure solution method	MIRAS
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Phasing software	SnB
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.071	0.170
Number of reflections	33400
<I/σ(I)>	15	5
Completeness [%]	97.5	96.3
Redundancy	5.8	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		28% PEG-400 0.2M CACL2 HEPES-NA BUFFER, PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG400	28 (%(v/v))
3	1	reservoir		0.2 (M)
4	1	reservoir	sodium HEPES	0.1 (M)