1QE0
CRYSTAL STRUCTURE OF APO S. AUREUS HISTIDYL-TRNA SYNTHETASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-11-24 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 65 |
| Unit cell lengths | 125.650, 125.650, 115.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.700 |
| R-factor | 0.192 |
| Rwork | 0.197 |
| R-free | 0.27000 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.000 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.194 | 0.564 |
| Total number of observations | 136360 * | |
| Number of reflections | 28925 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 298 | Citrate, Tartrate, (NH4)2SO4, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 7 | 1 | reservoir | sodium potassium tartrate | 0.2 (M) | |
| 8 | 1 | reservoir | ammonium sulfate | 1.8 (M) |
