1QDN
AMINO TERMINAL DOMAIN OF THE N-ETHYLMALEIMIDE SENSITIVE FUSION PROTEIN (NSF)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-11 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 101.900, 101.900, 126.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.26000 |
RMSD bond length | 0.006 |
RMSD bond angle | 24.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (0.5) |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.058 | 0.312 |
Number of reflections | 28518 | |
<I/σ(I)> | 19.6 | |
Completeness [%] | 98.9 | 98.7 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * | TRIS, AMMONIUM SULPHATE, 2- MERCAPTOETHANOL, pH 8.2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 28 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 25 (mM) | |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | 2-mercaptoethanol | 10 (mM) | |
5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 1.85-2.05 (M) | |
7 | 1 | reservoir | 2-mercaptoethanol | 10 (mM) |