1QD9
Bacillus subtilis YABJ
Experimental procedure
| Experimental method | MULTIPLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-07-11 |
| Detector | ADSC QUANTUM 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 53.350, 53.350, 204.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.19700 |
| Structure solution method | HG MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.053 | 0.086 |
| Number of reflections | 35377 * | |
| <I/σ(I)> | 22.9 | 8.6 |
| Completeness [%] | 97.9 | 95.6 * |
| Redundancy | 8.7 | 4.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | SODIUM PHOSPHATE, PEG 4000, AMMONIUM SULPHATE, SODIUM ACETATE., pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.3 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 40 (mM) | pH7.4 |
| 3 | 1 | reservoir | PEG4000 | 20 (%) | |
| 4 | 1 | reservoir | ammonium acetate | 0.45 (M) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
