1QD2
CRYSTAL STRUCTURE OF THE COMPLEX OF TRICHOSANTHIN WITH ADENINE, OBTAINED FROM TRICHOSANTHIN COMPLEXED WITH THE DINUCLEOTIDE APG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.410, 76.720, 79.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.860 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| R-free | 0.22100 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.266 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.900 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.078 | 0.343 |
| Number of reflections | 20045 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 98.8 | 90.3 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 20 * | HANGING DROP, CITRATE BUFFER, POTASSIUM CHLORIDE, DINUCLEOTIDE APG. NATIVE CRYSTALS SOAKED IN SOLUTION CONTAINING DINUCLEOTIDE APG, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | citrate | 0.004 (M) | |
| 3 | 1 | drop | corresponding substrates | 1-2 (mg/ml) | |
| 4 | 1 | drop | 7 (%) | ||
| 5 | 1 | reservoir | 14 (%) | ||
| 6 | 1 | reservoir | sodium citrate | 0.008 (M) |
