1QD0
CAMELID HEAVY CHAIN VARIABLE DOMAINS PROVIDE EFFICIENT COMBINING SITES TO HAPTENS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-04-29 |
| Detector | OTHER |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 46.700, 46.700, 121.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 2.500 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.28000 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 27.000 * |
| Data reduction software | DENZO |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.300 | 2.500 |
| Rmerge | 0.060 | 0.250 |
| Total number of observations | 57351 * | |
| Number of reflections | 8364 * | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 96.0 | 62 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | ammonium sulfate, sodium acetate, glycerol, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 20.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | hapten | 5 (mM) | |
| 2 | 1 | drop | VHH | 11 (mg/ml) | |
| 3 | 1 | drop | Tris-HCl | 10 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.3 (M) | |
| 5 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 6 | 1 | reservoir | glycerol | 6-7 (%) |
