1QCU
CRYSTAL STRUCTURE OF AN 18 BASE PAIR COPY CONTROL RELATED RNA DUPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-05-17 |
Detector | PRINCETON 2K |
Spacegroup name | P 3 |
Unit cell lengths | 43.806, 43.806, 29.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.200 |
R-factor | 0.116 * |
R-free | 0.15000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | A-RNA |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.930 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.073 | 0.274 |
Number of reflections | 18266 | |
<I/σ(I)> | 31.8 | 5.2 |
Completeness [%] | 93.0 * | 77 * |
Redundancy | 4.2 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 20 * | (NH4)2SO4, SODIUM CACODYLATE, MGCL2, ROP PROTEIN, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | Vapor diffusion * | 6.5 | 20 * | (NH4)2SO4, SODIUM CACODYLATE, MGCL2, ROP PROTEIN, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2SO4 | ||
2 | 1 | 1 | SODIUM CACODYLATE | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | ROP PROTEIN | ||
5 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | RNA oligo | 1.0 (mM) | |
2 | 1 | drop | rop protein | 1.0 (mM) | |
3 | 1 | drop | ammonium sulfate | 1.05 (M) | |
4 | 1 | drop | sodium cacodylate | 5 (mM) | |
5 | 1 | drop | 1 (mM) | ||
6 | 1 | drop | sodium azide | 0.02 (%) | |
7 | 1 | reservoir | ammonium sulfate | 2.1 (M) |